Level 1 – AI-based Humanization
The first module of humanization achieves in silico humanization of each antibody candidate. Once completed, up to 16 sequences will be generated with properties predicted and critical residues/liabilities annotated.
Detailed report will be provided with all antibody variants and associated data.
Level 2 – Micro-scale Production
In the second step of the pipeline, small production of antibodies will allow hands-on measurment of antibody properties. To generate quality data, we use HPLC-SEC, iQue®3 (HTP-Flow Cytometer) and ELISA assays.
Measurements include:
Production Yields
VH/VL pairing efficiency
Aggregation
Fragmentation
These properties are excellent predictors of antibody developability, manufacturability, solubility and stability.
Level 3 – Binding Strength and Specificity
Pipeline to ensure antibody binding and specificity will be developped according to the client’s needs and goals. Detailed report will be provided.
Binding strength and specificity measure by ELISA or flow cytometry.
Level 4 – High-Resolution Kinetic analysis
High-resolution kinetic curves can be generated for selected antibodies, ensuring complete characterization of antibody variants.
Instruments Available
Acuity Arc HPLC (HPLC-SEC)
- Assess protein purity and stability
- Up to 96 proteins per day
iQue®3 (HTP-Flow Cytometer)
- Assess binding and blocking capacities on cells or beads
- Up to 384 samples in 30 minutes
- 3 lasers (VBR) & 15 detection channels
- Low sample / time consumption
- Different kits available
Sony SH800S (FACS)
- Assess binding and blocking capacities on cells or beads
- Cell sorting into tubes or 384 well plates (SH800S)
- 4 lasers (VYBR) & 8 detection channels (SH800S)
Carterra LSA (HTP-SPR) & Octet Red 384 (BLI)
- Obtain binding kinetics (KD,ka,kd)
- Up to 1152 clones (SPR) & 384 (BLI) per run
- Epitope binning (384×384 pairwise interactions)
- Epitope mapping
- Screening of purified or crude protein extracts
- Low sample / time consumption (SPR)
- Up to 1600 proteins per week
