Overview

Expert services for antibody discovery, engineering, and protein optimization to accelerate your research

Available Antibody Services

Each element of our antibody discovery pipeline is offered as a standalone service, tailored to your specific needs.

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Multi-Approach Immunization

  • Diverse antigen representation
  • Potentializing immune repertoire

Macrocyclic Peptides (protein epitope mimetic)

Macrocyclic peptide used for immunization in our antibody discovery pipeline

Linear Peptides

Linear peptide used for immunization in our antibody discovery pipeline

DNA & RNA

DNA used for immunization in our antibody discovery pipeline

Proteins

Protein used for immunization in our antibody discovery pipeline

Cells

Cell used for immunization in our antibody discovery pipeline

Pseudo-Viral Particles

Pseudo-viral particle used for immunization in our antibody discovery pipeline

We offer 3 different selection methods that can be applied to almost any antibody format and perfomed in parallel if necessary

Phage Display

Yeast Display

Single B Cell
Expression System

Prokaryote

Eukaryote

Eukaryote
Library Size

1011-12

109

Unlimited
Throughput Per Day

Unlimited

1 billion

300k-600k
Complex Antigens

No

Yes

Yes
Antibody Formats

scFv, VHH, Fab (challenging)

scFv, VHH, Fab, IgG

IgY/IgG (secreted)
Specificity and Affinity

+

++

++
Rare Binders

Difficult

Yes

Yes

VH/VL

Random

Random

Natively paired

Developability

Variable

Good

Excellent
Phage display antibody selection analyzed with SPR in our antibody discovery pipeline
Phage Display
Yeast display antibody selection analyzed with flow cytometry in our antibody discovery pipeline
Yeast Display
Single B cell antibody selection analyzed by fluorescent imaging in our antibody discovery pipeline
Single B cell

Stable Pools

see Biomanufacturing page

Transient Expression

  • CHO
  • HEK

Production size tailored to the needs of the client (96 well-plates to 2L shake flasks)

Protein purification

  • Affinity chromatography (Protein A/G/L, custom immunoaffinity)
  • Size exclusion chromatography (SEC)
  • Ion-exchange chromatography (CEX, AEX)
  • Multi-column sequential chromatography

FPLC Systems

  • ÄKTA Avant 150
  • Bio-Rad NGC

Tangential Flow (TFF) and Viral Filtration

  • ÄKTA Flux S
  • ÄKTA Flux 6
  • Kross Flow
  • Bulk properties (appearance, conductivity, pH, osmolality, concentration)
  • Purity (aggregation, charge variants, size variants, impurity)
  • Safety (endotoxins, HCP, DNA, sterility)
  • Potency (target binding, cell-based assays)

Antibody Formats

  • ScFv
  • Nano-TCR
  • IgG1
  • IgG4
  • IgG2a
  • IgA
  • Chimeric chicken-human
  • Chimeric mouse-human
  • Chimeric human-mouse
  • Fc-fusion
  • IgG-scFv
  • Custom antibody fusion proteins

Humanization

Process of antibody humanization: from chicken IgY to chimeric antibody to humanized IgG

Bispecific and Multispecific Antibodies

Engineering a bispecific antibody with knob-into-hole technology by combining two monospecific antibodies for therapeutic applications

Sequence Optimization

The amino acid sequence of the critical regions for antibody affinity and developability is modified and optimized according to the following criteria :

Developability

  • Production yields
  • Product quality

Affinity

  • Affinity and kinetics analysis by SPR

Sequences are sorted based on improvement of affinity and production potential in order to provide a tailored optimal sequence

SPR

We offer small- and large-scale SPR analyses pipeline options for multiple antibody formats, either purified or crude extracts:

  • Affinity
  • Kinetics
  • Binning
  • Functional assays
SPR-based antibody selection workflow with Carterra LSA kinetic analysis leading to functional assay validation

Immune Biosolutions offers isolation and purification of polyclonal IgY antibodies with high affinity. Our polyclonal antibodies can recognize any target and are ideal for any diagnostic or detection reagents. Polyclonal projects can be pursued as recombinant monoclonal discovery project if requested.

Carterra LSA (HTP-SPR) & Octet Red 384 (BLI)

  • Obtain binding kinetics (KD,ka,kd)
  • Up to 1152 clones (SPR) & 384 (BLI) per run
  • Epitope binning (384×384 pairwise interactions)
  • Epitope mapping
  • Screening of purified or crude protein extracts
  • Low sample / time consumption (SPR)
  • Up to 1600 proteins per week
Carterra LSA machine for SPR
Octet Red 384 for Biolayer Interferometry (BLI)

Acuity Arc HPLC (HPLC-SEC)

  • Assess protein purity and stability
  • Up to 96 proteins per day
HPLC SEC instrument
HPLC SEC instrument

CellCelector (Single B Cell)

  • Single B-cells screening
  • Cell lines development
  • Protein production stable cell lines
  • CRISPR KO cell lines
  • Clonal cell lines
CellCelector instrument for Single B Cell selection or clonal selection

Biomek i7 (Automated Liquid Handler)

  • For large scale and precise liquid handling
  • Equipped with a centrifuge, PCR machines, plate shaker, plate heater, tips washing station and Cytomat 2 hotel (can store up to 42 multiwell plates)
  • 45 deck positions
  • 300 uL 96 well multichannel & span-8 pod (up to 1200 uL)
Biomek i7 Automated Liquid Handler

iQue®3 (HTP-Flow Cytometer) & Sony SH800S (FACS)

  • Assess binding and blocking capacities on cells or beads
  • Cell sorting into tubes or 384 well plates (SH800S)
  • 4 lasers (VYBR) & 8 detection channels (SH800S)
  • Up to 384 samples in 30 minutes (iQue®3)
  • 3 lasers (VBR) & 15 detection channels (iQue®3)
  • Low sample / time consumption (iQue®3)
  • Different kits available for iQue®3:
    • Antibody characterization
    • Cytokine profiling
    • Immune cell function
iQue3 HTP-Flow Cytometer
Sony SH800S FACS instrument