Monoclonal Antibodies
Phage Display |
Yeast Display |
Single B-Cell |
|
Expression System |
Prokaryote |
Eukaryote |
Eukaryote |
Library Size |
1011-12 |
109 |
Unlimited |
Throughput Per Day |
Unlimited |
1 billion |
300k-600k |
Complex Antigens |
To be discussed |
Yes |
Yes |
Antibody Formats |
scFv, VHH, Fab (challenging) |
scFv, VHH, Fab, IgG |
IgY/IgG (secreted) |
Specificity and Affinity |
++ |
+++ |
+++ |
Rare Binders |
Difficult |
Yes |
Yes |
VH/VL |
Random |
Random |
Natively paired |
Developability |
Variable |
Good |
Excellent |
Single B-Cell
Paired Light and Heavy Chains
Our Single Cell selection technology allows for rapid visual selection of antibody secreting cells using fluorescent microscopy.
IgG and IgY
Initial screen is performed with naturally secreted antibody format which is IgG (human and mouse) and IgY (chicken).
Multiple Targets and Cross-Reactivity
Assessment of antibody specificity can be assessed for multiple targets at the same time, in the same well.
Single Cell Picking
Once identified, antibody secreting cells of interest are isolated to identify heavy and light chain sequences.
This process is fast to allow maximum cell viability and sequence quality
Complex Antigens
Single Cell facilitates Selection on complex antigens
Yeast Display
scFv / VHH / Fab / IgG
Yeast display selection can be performed on many different antibody formats, customized to the client’s wishes.
Specificity and Affinity
Initial yeast display analysis with FACS allows for precise selection of antibodies that are well expressed and bing the target of interest with high specificity.
The choice of candidates can also be modulated ton include more or less candidates based on the strength of the signal.
Rare Binders
Yeast display allows for indivdual event selection and therefore allows for selection of rare events.
Eukaryotic Protein Folding
Yeast typically display properly folded proteins which further improves initial binder selection.
High-Throughput Cell Sorting
Cell Sorting in 384-well plates allows for single-cell sorting and analysis of a large number of candidates
PacBio Sequencing
Single-cell long-read sequencing using PacBio technology allows for final identification of candidate binders identified through FACS
Phage Display
High-Throughput
Phage display selection allows for highest throughput of all selection methods. For large pools of potential candidate binders for further characterization, using phage to display antibodies is the most effective method.
scFv / VHH / Fab
Phage display selection can be performed mainly on scFv and VHH. Fab is also possible but more challenging.
SPR and NGS selection
Further combination of high-throughput characterization of binders using next-generation-sequencing (NGS) and SPR (Carterra LSA) supports for rapid and thorough selection of large candidate libraries.
Discover our antibody services and contact us for more details!
Available Immunized Libraries
Immunized libraries are ready to use for fast-tracked antibody discovery campaigns.
LILRB1
LILRB2
PD-1
PD-L1
HLA-A
HLA-B
Neurotensin
Bradikinin
Kallidin
B1R
NTSR1
SARS-CoV-2 S1
SARS-CoV-2 S2
SARS-CoV-2 RBD
TGFβ-1
TGFβ-2
IL-1β
IL-25
IL-16
CD37
CCR8
Apelin
Carterra LSA (HTP-SPR)
- Binding kinetics (KD,ka,kd)
- Up to 1152 clones per run
- Epitope binning (384×384 pairwise interactions)
- Epitope mapping
- Screening of purified or crude protein extracts
- Low sample / time consumption
- Up to 1600 proteins per week
Octet® RED384
- Binding kinetics (KD,ka,kd)
- Up to 384 clones per run
- Epitope binning (384×384 pairwise interactions)
- Epitope mapping
- Screening of purified or crude protein extracts
CellCelector
- Single B-cells screening
- Cell lines development
- Protein production stable cell lines
- CRISPR KO cell lines
- Clonal cell lines
Sony SH800S (FACS)
- Assess binding and blocking capacities on cells or beads
- Cell sorting into tubes or 384 well plates
- 4 lasers (VYBR) & 8 detection channels
