Antibody and Protein Production

At Immune Biosolutions, we can produce a large array of antibody and protein formats. Contact us for a customized production pipeline.

Expression, Production and Purification

Immune Biosolutions has a GMP and non-GMP manufacturing facility for antibody and recombinant protein production. We offer protein expression and purification services including all upstream and downstream processes necessary to bring a protein compound of interest to pre-clinical and clinical studies.

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Stable Pools and Cell Banking

see Biomanufacturing page

Transient Expression

CHO and HEK cells used routinely

Production size tailored to the needs of the client (96 well-plates to 2L shake flasks)

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Purification

Protein purification

  • Affinity chromatography (Protein A/G/L, custom immunoaffinity)
  • Size exclusion chromatography (SEC)
  • Ion-exchange chromatography (CEX, AEX)
  • Multi-column sequential chromatography

FPLC Systems

  • ÄKTA Avant 150
  • Bio-Rad NGC

Tangential Flow (TFF) and Viral Filtration

  • ÄKTA Flux S
  • ÄKTA Flux 6
  • Kross Flow
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Physico-Chemical Characterization

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  • Bulk properties (appearance, conductivity, pH, osmolality, concentration)
  • Purity (aggregation, charge variants, size variants, impurity)
  • Safety (endotoxins, HCP, DNA, sterility)
  • Potency (target binding, cell-based assays)

Antibody and Protein Formats

  • ScFv
  • Nano-TCR
  • IgG1
  • IgG4
  • IgG2a
  • IgA
  • Chimeric chicken-human
  • Chimeric mouse-human
  • Chimeric human-mouse
  • Fc-fusion
  • IgG-scFv
  • Custom antibody fusion proteins

Contact us for more details on the custom antibody formats

Antibody and Protein Production
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